Human synexin (annexin VII) polymorphisms: tissue specificity and expression in Escherichia coli.
نویسندگان
چکیده
A. LEE BURNS,* KARIN MAGENDZO,* MEERA SRIVASTAVA,t EDUARDO ROJAS,* CLAUD0 PARRA,* MILTON DE LA FUENTE,* CONSTANCE CULTRARO,* ANAT SHIRVAN,* TIKVA VOGEL.* JUDY HELDMAN,* HUNG CAOHUY.* DONATELLA TOMBACCINI* and HARVEY B. POLLARD* * Luhorutoty of Cell Bioloby utid Geiietits, Nutiotiul ltistitiite of1)iuhete.s utid Digestive utid Kidtiey Diseuses, Nutionul 1ti.stitute.s of lleulth. Bethesdu, M D 20892, U.S.A. utid t Curdioretiul Ilivisioti, Food utid Dnig Adrnitiistrutioti, Bethesdu, M I ) 2(uI92, U.S.A.
منابع مشابه
Alternative splicing of human synexin mRNA in brain, cardiac, and skeletal muscle alters the unique N-terminal domain.
Several synexin (annexin VII) mRNAs have been identified by screening a human fibroblast cDNA library. One type of message contained an alternatively spliced cassette exon, predicting two isoforms of synexin differing in the N-terminal domain. Polymerase chain reaction analysis of synexin mRNA from various fetal and adult tissues, from human and monkey, revealed that the alternative splicing ev...
متن کاملExpression and localization of annexin VII (synexin) in muscle cells.
Annexin VII (synexin) is a member of the annexin family of proteins, which are characterized by Ca(2+)-dependent binding to phospholipids. We used PCR to isolate from a lambda gt11-mouse fibroblast library annexin VII cDNA fragments corresponding to the two isoforms found in both humans and Dictyostelium discoideum. The two isoforms of 47 kDa and 51 kDa differed by 22 amino acids inserted into ...
متن کاملMouse synexin (annexin VII) polymorphisms and a phylogenetic comparison with other synexins.
Two sets of cDNAs encoding mouse synexin were isolated from a liver cDNA library and sequenced. The coding regions of synexin clones show 99% identity. By contrast, the two mouse synexin cDNAs differ in a number of ways in both 5' and 3' non-coding regions. The two sets of cDNA encode a polypeptide of 463 amino acid residues which has a deduced molecular mass of 50 kDa. The amino acid sequence ...
متن کاملEffects of the expression of mammalian annexins in yeast secretory mutants.
The hypothesis that calcium-dependent membrane-binding proteins of the annexin family can influence intracellular membrane trafficking was tested by expressing five mammalian annexins in wild-type yeast cells (Saccharomyces cerevisiae) and in 13 yeast secretory (sec) mutants. Expression of human synexin (annexin VII) inhibited the growth of sec2, sec4 and sec15 mutants at a semi-permissive temp...
متن کاملCalcium-dependence of synexin binding may determine aggregation and fusion of lamellar bodies.
Synexin (annexin VII) is a member of the annexin family of calcium and phospholipid binding proteins that promote calcium-dependent aggregation and fusion of lipid vesicles or secretory granules. We have previously suggested that synexin may be involved in membrane fusion processes during exocytosis of lung surfactant since it promotes fusion in vitro of lamellar bodies with plasma membranes. I...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 18 6 شماره
صفحات -
تاریخ انتشار 1990